This study identified factors affecting the practice of standard precaution among health workers’ in government owned hospitals. The design of this study was descriptive survey and the target population was health workers. Purposive sampling technique was used to select 132 respondents and a self-developed questionnaire was the instrument used to collect data which was analysed using descriptive and inferential statistics tools such as frequency distribution, percentages, tables and Pearson product moment correlation through the statistical package for social sciences (SPSS version 23). The results revealed thatsupply of personal protective equipment and uncomfortable/obsolete equipment were factors affecting the practice of standard precaution among health care workers. It further revealed that inadequate knowledge, time shortage, forgetfulness and inadequate training were not factors affecting the practice of  standard precaution among health care workers. Also, significant relationship was found between lack of time and practice of standard precaution among health care workers. It was observed that there is significant relationship between forgetfulness and practice of standard precaution among health care workers.It was therefore recommended that adequate preferences should be given to health workers by displaying chart, board and art-work that shows images and pictures of practice of standard precaution in the wards and offices of health workers to serve as continuous reminder to them on the importance and health benefits of practice of standard precaution.